How the Diesel Testing Kit Works
Diesel Testing Kit with Lateral Flow Device Technology:
Authentix’ breaktrough, patented, antibody-based technology was developed to recognise any form of illicit mixing of Diesel with Illuminating Paraffin.
How to use a diesel test kit:
1. Obtain sample of diesel fuel to be tested
2. Open the 2ml micro tube and empty the solution into the provided mixing bottle
3. Fill the mixing bottle to the neck with sampled diesel fuel using the pipette
4. Using the small cone on the provided closure, cork the mixing bottle and, while holding closed, shake vigorously for 60 seconds
5. Place mixing bottle on flat surface and allow to settle until the sample has visibly separated into two layers. Wait at least 5 minutes for sufficient separation between the extraction buffer and fuel. Failure to wait for 5 minutes may produce inaccurate or invalid results
6. Remove the LFD from package
7. Uncork the mixing bottle and securely screw on the dropper bottle cap
8. Gently invert the mixing bottle and discard the first two drops, and thereafter, while keeping the mixing bottle inverted, apply the next 5 drops to the LFD
9. Allow the LFD to develop for 5 minutes
- Line at C position only (1 Line) – Indicates IP Present.
- Line at C&T position (2 Lines) -Indicates IP Absent.
- Note: The C line acts as a control and should always develop. In the unlikely event that this does not occur, the device has failed, and the test should be repeated using a fresh device.
How Paraffin is detected in Diesel?
When Diesel is tested any Illuminating Paraffin (containing the South African Revenue Service – Marker) mixed into the Diesel will cause antibodies to be released and bind specifically to the marker in the sample. A line (1 line) at the C position will appear on the diesel test kit indicating that the Diesel has been mixed with Illuminating Paraffin.
How will you recognize clean Diesel?
When Diesel is tested that has not been adulterated/mixed with Illuminating Paraffin the antibody/sample solution is drawn across the membrane. As there is no marker present in the sample, the antibodies are still free to bind to the marker which is laid down. Lines (2 lines) at the C&T position will appear on the diesel testing kit indicating that the Diesel is clear and has not been adulterated with Illuminating Paraffin.